ku７０/ku８０のヘテロダイマーにDNA―PKcs が結合し、DNA―PKcs が活性 化します。その後、artemis によってDNA 切断端を修飾し、ligaxe IV とXRCC４
Ku70 is required for late B cell development and immunoglobulin heavy chain class switching Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement.
This was probably caused by the much slower growth of the Ku70 shRNA cells (F.J.F., unpublished observations), which may have a deleterious effect on gene targeting because rAAV preferentially transduces actively dividing Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. The Ku70‐specific C‐terminal region consists of a 5 kDa SAP domain (Ku70‐SAP), the structure of which was determined both in isolation (Zhang et al, 2001) and as part of the Ku70–Ku80 crystal structure (Walker et al, 2001; Fig 1C). Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation.
Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects. Ku70 Antibody (MA5-32645) in WB Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31) (Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination.
XRCC6 (X-Ray Repair Cross Complementing 6) is a Protein Coding gene. Diseases associated with XRCC6 include Lupus Erythematosus and Nijmegen Breakage Syndrome.Among its related pathways are Cytosolic sensors of pathogen-associated DNA and DNA damage_NHEJ mechanisms of DSBs repair.Gene Ontology (GO) annotations related to this gene include protein C-terminus binding.
Securin 2021-02-22 · Acetylation modulates DNA-mediated Ku70-dependent IFN-λ1 induction. Ku80 colocalizes with Ku70 during Ku70 translocation from the nucleus to the cytoplasm, but not directly involving in Ku70's cytosolic DNA activity.
Anti-Ku70 Antibody (E-5) is a mouse monoclonal IgG 1 κ Ku70 antibody, cited in 60 publications, provided at 200 µg/ml; raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin
Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. Royaltyn eller avgiften ska då i stället redovisas på KU10. Att royaltyn ska redovisas som tjänst gäller däremot inte om den som fått royaltyn inte ska betala skatt i Sverige och inte heller omfattas av svensk socialförsäkring.
KU70 – Kontrolluppgift – Näringsbidrag och royalty. Denna blankett ska användas av den som ska lämna kontrolluppgift avseende näringsbidrag och royalty. Ku70 and Ku80 have also been experimentally characterized in plants, where they appear to play a similar role to that in other eukaryotes.
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Visit Bethyl.com for all your antibody needs. Ku70 and Ku80 participate in LPS-induced pro-inflammatory cytokines production in human macrophages and monocytes. Hong Sun 1, *, , Quan Li X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 6. THYROID AUTOANTIGEN, 70-KD; G22P1 Ku ANTIGEN, 70-KD SUBUNIT; Ku70 The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA Nov 19, 2019 Moreover, chromatin recruitment of key NHEJ proteins, including, Ku70, Ku80, DNA-PKcs and XLF was diminished in autophagy-deficient cells.
Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL).
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Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA.
Fig. 1. Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice. (A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm. (B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation of ubiquitylated Bax. Under normal growth conditions, Bax ubiquitylation The protein of 70-kDa molecular weight is a DNA-helicase involved in the DNA double-strand break repair system (Double-Strand Break DNA Repair), more precisely the nonhomologous end joining.
Jun 1, 2001 Background and Purpose—Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks,
The Ku70‐specific C‐terminal region consists of a 5 kDa SAP domain (Ku70‐SAP), the structure of which was determined both in isolation (Zhang et al, 2001) and as part of the Ku70–Ku80 crystal structure (Walker et al, 2001; Fig 1C). Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation. Ku is Knockdown of Ku70 was achieved by transfecting HeLa with Ku70 specific siRNAs (Silencer® select Product # S5455, S5457). Western blot analysis (Fig.
Royaltyn eller avgiften ska då i stället redovisas på KU10. Att royaltyn ska redovisas som tjänst gäller däremot inte om den som fått royaltyn inte ska betala skatt i Sverige och inte heller omfattas av svensk socialförsäkring. I de fallen ska royaltyn alltid redovisas på KU70.